Molecular weight (DA)
46kD
Immunogen
The antiserum was produced against synthesized peptide derived from human BCKD . AA range:11-60
Specificity
BCKDK Polyclonal Antibody detects endogenous levels of BCKDK protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012],
Function
catalytic activity:ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate.,function:Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.,PTM:Autophosphorylated.,similarity:Belongs to the PDK/BCKDK protein kinase family.,similarity:Contains 1 histidine kinase domain.,subunit:Monomer.,tissue specificity:Ubiquitous.,
Protein name
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial
Other name
BCKDK; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN
Human protein sequence Database
O14874
Mouse protein sequence database
O55028
Rat protein sequence database
Q00972
Cellular localization
Mitochondrion matrix. Mitochondrion .
Tissue expression
Ubiquitous.
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