Molecular weight (DA)
96kD
Immunogen
The antiserum was produced against synthesized peptide derived from human TOP3B. AA range:51-100
Specificity
Topo IIIβ-1 Polyclonal Antibody detects endogenous levels of Topo IIIβ-1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013],
Function
catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,function:Possesses negatively supercoiled DNA relaxing activity.,similarity:Belongs to the prokaryotic type I/III topoisomerase family.,tissue specificity:Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.,
Protein name
DNA topoisomerase 3-beta-1
Other name
TOP3B; TOP3B1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1
Fields
>>Homologous recombination;>>Fanconi anemia pathway
Human protein sequence Database
O95985
Mouse protein sequence database
Q9Z321
Rat protein sequence database
Cellular localization
condensed chromosome,nucleus,cytosol,
Tissue expression
Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.
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