Molecular weight (DA)
55kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Nuclear Factor 1. AA range:11-60
Specificity
NF-1 Polyclonal Antibody detects endogenous levels of NF-1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Function
function:Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the CTF/NF-I family.,similarity:Contains 1 CTF/NF-I DNA-binding domain.,subunit:Binds DNA as a homodimer.,
Protein name
Nuclear factor 1 A-type
Other name
NFIA; KIAA1439; Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein; NFIB; Nuclear factor 1 B-type; NF1-B; Nuclear factor 1/B; CCAAT-box-b
Human gene ID
4774/4781/4782/4784
Human protein sequence Database
Q12857/O00712/P08651/Q14938
Mouse gene ID
18027/18028/18029/18032
Mouse protein sequence database
Rat protein sequence database
P09414
Cellular localization
Nucleus.
Tissue expression
Brain,Epithelium,Skeletal muscle,Testis,
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