Molecular weight (DA)
65kD
Immunogen
Synthesized peptide derived from Eme1 . at AA range: 250-330
Specificity
Eme1 Polyclonal Antibody detects endogenous levels of Eme1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009],
Function
cofactor:Magnesium.,function:Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.,similarity:Belongs to the EME1/MMS4 family.,subcellular location:Recruited to regions of DNA damage in S-phase cells.,subunit:May self-associate. Interacts with MUS81. Interacts with ERCC4 and FANCM.,
Protein name
Crossover junction endonuclease EME1
Other name
EME1; MMS4; Crossover junction endonuclease EME1; MMS4 homolog; hMMS4
Fields
>>Homologous recombination;>>Fanconi anemia pathway
Human protein sequence Database
Q96AY2
Mouse protein sequence database
Q8BJW7
Rat protein sequence database
Cellular localization
Nucleus, nucleolus . Recruited to regions of DNA damage in S-phase cells.
Tissue expression
Epithelium,Placenta,
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