55kD

The antiserum was produced against synthesized peptide derived from human CD3EAP. AA range:441-490

CD3EAP Polyclonal Antibody detects endogenous levels of CD3EAP protein.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

1 mg/ml

caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling.,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.,function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.,miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function.,PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.,PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eukaryotic RPA34 RNA polymerase subunit family.,subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.,

caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling.,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.,function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.,miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an

CD3EAP

DNA-directed RNA polymerase I subunit RPA34

CD3EAP

CD3EAP; ASE1; CAST; PAF49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49

>>RNA polymerase

10849

O15446

Q76KJ5

Nucleus, nucleolus . Chromosome . Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.

Eye,Serum,Skin,Testis,

-20°C/1 year