Molecular weight (DA)
31kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H1 around the phosphorylation site of Thr17. AA range:1-50
Specificity
Phospho-Histone H1 (T17) Polyclonal Antibody detects endogenous levels of Histone H1 protein only when phosphorylated at T17.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
Function
function:Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.,similarity:Belongs to the histone H1/H5 family.,
Protein name
Histone H1.5
Other name
HIST1H1B; H1F5; Histone H1.5; Histone H1a; Histone H1b; Histone H1s-3; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4
Human gene ID
3009/3007/3008
Human protein sequence Database
P16401/P16402/P10412
Mouse gene ID
56702/14957/50709
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Nucleus. Chromosome. According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.
Tissue expression
Ubiquitous. Expressed in the majority of the cell lines tested and in testis.
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