Molecular weight (DA)
55kD
Immunogen
The antiserum was produced against synthesized peptide derived from human FOXO4 around the phosphorylation site of Thr451. AA range:417-466
Specificity
Phospho-FoxO4 (T451) Polyclonal Antibody detects endogenous levels of FoxO4 protein only when phosphorylated at T451.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010],
Function
disease:A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.,function:Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.,pharmaceutical:A constitutively active FOXO4 mutant where phosphorylation sites Thr-32, Ser-187 and Ser-262 have been mutated to alanine may have therapeutic potential in ERBB2/HER2-overexpressing cancers as it inhibits ERBB2-mediated cell survival, transformation and tumorigenicity.,PTM:Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Dea
Protein name
Forkhead box protein O4
Other name
FOXO4; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1
Fields
>>Ras signaling pathway;>>FoxO signaling pathway;>>Shigellosis
Human protein sequence Database
P98177
Mouse protein sequence database
Q9WVH3
Rat protein sequence database
Cellular localization
Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
Tissue expression
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
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