Immunogen
The antiserum was produced against synthesized peptide derived from human NF-kappaB p100/p52 around the phosphorylation site of Ser869. AA range:836-885
Specificity
Phospho-NFκB-p100 (S869) Polyclonal Antibody detects endogenous levels of NFκB-p100 protein only when phosphorylated at S869.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
Function
disease:A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant.,disease:A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT).,disease:In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.,domain:The C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52 homodimers, and/or transcription activation.,domain:The glycine-rich region (GRR) appears to be a critical element in the generation of p52.,function:NF-kappa-B is a pleiotropic transcription factor which is present in almost a
Protein name
Nuclear factor NF-kappa-B p100 subunit
Abbreviation
NF-κB p100/p52
Other name
NFKB2; LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10
Fields
>>MAPK signaling pathway;>>NF-kappa B signaling pathway;>>Osteoclast differentiation;>>C-type lectin receptor signaling pathway;>>Legionellosis;>>Human T-cell leukemia virus 1 infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Breast cancer
Human protein sequence Database
Q00653
Mouse protein sequence database
Q9WTK5
Rat protein sequence database
Cellular localization
Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
Tissue expression
Leukemia,Lymph,Thymus,
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