Molecular weight (DA)
14kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys12. AA range:10-59
Specificity
Acetyl-Histone H2B (K12) Polyclonal Antibody detects endogenous levels of Histone H2B protein only when acetylated at K12.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
H2BFS (H2B Histone Family Member S (Pseudogene)) is a Pseudogene. Diseases associated with H2BFS include endometrial stromal sarcoma. Among its related pathways are Packaging Of Telomere Ends. GO annotations related to this gene include sequence-specific DNA binding and protein heterodimerization activity. An important paralog of this gene is HIST1H2BH.
Function
DNA packaging, chromatin organization, chromatin assembly or disassembly, nucleosome assembly, defense response, response to bacterium, chromatin assembly, cellular macromolecular complex subunit organization, cellular macromolecular complex assembly, nucleosome organization, defense response to bacterium, macromolecular complex subunit organization, chromosome organization, macromolecular complex assembly, protein-DNA complex assembly,
Protein name
Histone H2B type F-S
Other name
H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s
Fields
>>Neutrophil extracellular trap formation;>>Alcoholism;>>Viral carcinogenesis;>>Systemic lupus erythematosus
Human protein sequence Database
P57053
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Nucleus. Chromosome.
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