Immunogen
The antiserum was produced against synthesized peptide derived from human DNA-PK around the phosphorylation site of Thr2647. AA range:2613-2662
Specificity
Phospho-DNA-PKCS (T2647) Polyclonal Antibody detects endogenous levels of DNA-PKCS protein only when phosphorylated at T2647.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Inhibited by wortmannin. Activity of the enzyme seems to be attenuated by autophosphorylation.,function:Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of
Protein name
DNA-dependent protein kinase catalytic subunit
Other name
PRKDC; HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460
Fields
>>Non-homologous end-joining;>>Cell cycle
Human protein sequence Database
P78527
Mouse protein sequence database
P97313
Rat protein sequence database
Cellular localization
Nucleus . Nucleus, nucleolus .
Tissue expression
Brain,Cervix carcinoma,Epithelium,Fetal lung,Placen
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