Immunogen
The antiserum was produced against synthesized peptide derived from human PTEN around the phosphorylation site of Ser385. AA range:370-400
Specificity
Phospho-PTEN (S385) Polyclonal Antibody detects endogenous levels of PTEN protein only when phosphorylated at S385.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate ener
Function
catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,catalytic activity:Phosphatidylinositol 3,4,5-trisphosphate + H(2)O = phosphatidylinositol 4,5-bisphosphate + phosphate.,catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,cofactor:Magnesium.,disease:A microdeletion of chromosome 10q23 involving PTEN and BMPR1A is a cause of chromosome 10q23 deletion syndrome [MIM:612242]. This syndrome shows overlapping features of the following three disorders: Bannayan-Zonana syndrome, Cowden disease and juvenile polyposis syndrome.,disease:Defects in PTEN are a cause of Bannayan-Zonana syndrome (BZS) [MIM:153480]; also known as Ruvalcaba-Riley-Smith or Bannayan-Riley-Ruvalcaba syndrome (BRRS). In BZS there seems not to be an increased risk of malignancy. It has a partial clinical overlap with CD. BZS is characterized by the classic triad of macroce
Protein name
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Other name
PTEN; MMAC1; TEP1; Phosphatidylinositol 3; 4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog
Fields
>>Inositol phosphate metabolism;>>Metabolic pathways;>>EGFR tyrosine kinase inhibitor resistance;>>FoxO signaling pathway;>>Phosphatidylinositol signaling system;>>Sphingolipid signaling pathway;>>p53 signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Cellular senescence;>>Focal adhesion;>>Insulin resistance;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Endometrial cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Central carbon metabolism in cancer;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Diabetic cardiomyopathy
Human protein sequence Database
P60484
Mouse protein sequence database
O08586
Rat protein sequence database
Cellular localization
Cytoplasm . Nucleus . Nucleus, PML body . Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies (PubMed:18716620). XIAP/BIRC4 promotes its nuclear localization (PubMed:19473982). .; [Isoform alpha]: Secreted . May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif.
Tissue expression
Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
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