Rat

0.625ng/ml - 20 ng/ml

0.1 ng/ml

Used to detect the concentration of rat neurotrophin receptor P75 (p75NTR) in samples such as serum, plasma, cell culture supernatant and tissue.

This kit recognizes native and recombinant rat neurotrophin receptor p75 (p75NTR) with no crosstalk with structural analogs.

The intra-batch coefficient of variation CV% was less than 10%; the inter-batch coefficient of variation CV% was less than 15%.

1. ELISA reader capable of detecting absorbance at 450 nm 2. Pipette and tip, sample loading tank 3. 37°C thermostat or water bath 4. Test tubes, centrifuge tubes, measuring cylinders, etc. for preparing reagents 5. Distilled water or deionized water

All reagents and components should be restored to room temperature first. For standards, quality control products and samples, duplicate wells are recommended. 1. Prepare the working solutions of various components of the kit according to the method described in the previous instructions. 2. Take out the required strips from the aluminum foil bag, seal the remaining strips with a ziplock bag and put them back in the refrigerator. 3. Set up standard wells, 0 value wells, blank wells and sample wells. Add 50μL of standards of different concentrations to the standard wells, 50μL of sample diluent to the 0 value wells, nothing to the blank wells, and 50μL of the sample to be tested to the sample wells. 4. In addition to the blank wells, add 100μL of horseradish peroxidase (HRP)-labeled detection antibody to the standard wells, 0 value wells and sample wells. 5. Cover the reaction plate with a sealing film and incubate in a 37°C water bath or incubator away from light for 60 minutes. 6. Remove the sealing film, discard the liquid, pat dry on absorbent paper, fill each well with washing solution, let stand for 20S, shake off the washing solution, pat dry on absorbent paper, and repeat this 5 times. If using an automatic plate washer, please wash the plate according to the operating procedures of the plate washer. Adding a soaking procedure for 30s can improve the accuracy of the test. After washing the plate, before adding the substrate, pat the reaction plate dry on a clean and non-chipping paper. (Tip: To obtain ideal experimental results, the residual liquid must be completely removed. After washing the plate, please proceed to the next step immediately and do not let the microplate dry.) 7. Mix substrate A and B in a 1:1 volume ratio and add 100μL of substrate mixture to all wells. Cover the reaction plate with a sealing film and incubate in a 37℃ water bath or incubator away from light for 15min. 8. Add 50μL of stop solution to all wells and read the absorbance (OD value) of each well on a 450nm wavelength microplate reader.