Rat

Negative - Positive

High sensitivity

Used to qualitatively detect whether rat Toxoplasma gondii IgG antibodies (Tox IgG) are present in samples such as serum, plasma, cell culture supernatant and tissue.

The kit uses an indirect enzyme-linked immunosorbent assay (ELISA). The specimen, negative and positive controls are added to the coated microwells pre-coated with rat HSV-I-IgG capture antigen, and then the HRP-labeled detection antibody is added. After incubation and thorough washing. The substrate TMB is used for color development. TMB is converted into blue under the catalysis of peroxidase and into the final yellow under the action of acid. The depth of color is positively correlated with the rat HSV-I-IgG in the sample. The absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader to determine the positive and negative.

This kit recognizes natural rat herpes simplex virus type I IgG antibody (HSV-I-IgG) and has no cross-reactivity with structural analogs.

See the instruction manual for details

The intra-batch coefficient of variation CV% was less than 10%; the inter-batch coefficient of variation CV% was less than 15%.

The recovery rate is between 85%-115%.

1. ELISA reader capable of detecting absorbance at 450 nm 2. Pipette and tip, sample loading tank 3. 37°C thermostat or water bath 4. Test tubes, centrifuge tubes, measuring cylinders, etc. for preparing reagents 5. Distilled water or deionized water

All reagents and components should be restored to room temperature. For standards, quality control products and samples, duplicate wells are recommended. 1. Prepare the working solutions of various components of the kit according to the method described in the previous instructions. 2. Take out the required strips from the aluminum foil bag, seal the remaining strips with self-sealing bags and put them back in the refrigerator. 3. Set up standard wells and sample wells, and add 50μL of different concentrations of standards to each standard well; 4. Add 50μL of the sample to be tested to the sample well; do not add to the blank well. 5. Except for the blank well, add 100μL of horseradish peroxidase (HRP) labeled detection antibody to each well of the standard well and sample well, seal the reaction well with a sealing film, and incubate at 37℃ water bath or constant temperature box for 60min. 6. Discard the liquid, pat dry on absorbent paper, fill each well with washing solution (350μL), let it stand for 1min, shake off the washing solution, pat dry on absorbent paper, and repeat the washing process 5 times (you can also use a plate washer to wash the plate). 7. Add 50 μL of substrate A and B to each well and incubate at 37°C in the dark for 15 min. 8. Add 50 μL of stop solution to each well and measure the OD value of each well at a wavelength of 450 nm within 15 min.