Monkey

0.25ng/mL - 8ng/mL

0.1ng/mL

Used to detect the concentration of monkey angiotensin II receptor 1 autoantibody (ATⅡR1-Ab) in samples such as serum, plasma, cell culture supernatant and tissue.

This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microplate pre-coated with anti-monkey ATⅡR1-Ab antibody (solid phase antibody), add monkey ATⅡR1-Ab calibrator and the sample to be tested, then add HRP-labeled anti-monkey ATⅡR1-Ab antibody (enzyme-labeled antibody). After incubation and sufficient washing, remove the unbound components, and form a sandwich complex of solid phase antibody-antigen-enzyme-labeled antibody on the solid surface of the microplate. Add substrates A and B, and the substrate produces a blue product under the catalysis of HRP. Under the action of the stop solution (acidic solution), it is finally converted into yellow. The absorbance (OD value) is measured at a wavelength of 450nm on an enzyme reader. The absorbance (OD value) is positively correlated with the concentration of monkey ATⅡR1-Ab in the sample to be tested. Fitting the calibration curve can calculate the concentration of monkey ATⅡR1-Ab in the sample.

This kit recognizes natural monkey angiotensin II receptor 1 autoantibody (ATⅡR1-Ab) and has no cross-reaction with structural analogs.

See the instruction manual for details

The intra-batch coefficient of variation CV% was less than 10%; the inter-batch coefficient of variation CV% was less than 15%.

The recovery rate is between 85%-115%.

1. ELISA reader capable of detecting absorbance at 450 nm 2. Pipette and tip, sample loading tank 3. 37°C thermostat or water bath 4. Test tubes, centrifuge tubes, measuring cylinders, etc. for preparing reagents 5. Distilled water or deionized water

All reagents and components should be restored to room temperature. For standards, quality control products and samples, duplicate wells are recommended. 1. Prepare the working solutions of various components of the kit according to the method described in the previous instructions. 2. Take out the required strips from the aluminum foil bag, seal the remaining strips with self-sealing bags and put them back in the refrigerator. 3. Set up standard wells and sample wells, and add 50μL of different concentrations of standards to each standard well; 4. Add 50μL of the sample to be tested to the sample well; do not add to the blank well. 5. Except for the blank well, add 100μL of horseradish peroxidase (HRP) labeled detection antibody to each well of the standard well and sample well, seal the reaction well with a sealing film, and incubate at 37℃ water bath or constant temperature box for 60min. 6. Discard the liquid, pat dry on absorbent paper, fill each well with washing solution (350μL), let it stand for 1min, shake off the washing solution, pat dry on absorbent paper, and repeat the washing process 5 times (you can also use a plate washer to wash the plate). (Tip: To obtain ideal experimental results, the residual liquid must be completely removed. After washing, please proceed to the next step immediately and do not let the microplate dry.) 7. Add 50μL of substrate A and B to each well and incubate at 37℃ in the dark for 15 minutes. 8. Add 50μL of stop solution to each well and measure the OD value of each well at a wavelength of 450nm within 15 minutes.